Presenter: Upasna Srivastava, PhD

Omics data is revolutionizing biological research, providing deeper insights into cellular diversity and function. Single-cell RNA sequencing (scRNA-seq) enables the study of gene expression at the individual cell level, offering a more detailed view of heterogeneity within tissues or organisms.

In this session, you will learn how to analyze 10X Chromium single-cell RNA-seq data using Seurat, a widely used R package for scRNA-seq analysis. This hands-on workshop will guide you through key steps in data processing, analysis, and visualization, helping you extract meaningful biological insights from your dataset.

You will learn how to:

• Import and preprocess 10X Genomics single-cell RNA-seq data in R.
• Perform quality control, normalization, and scaling to prepare data for analysis.
• Apply dimensionality reduction techniques (PCA, t-SNE, UMAP) to visualize cellular diversity.
• Identify distinct cell populations using clustering algorithms.
• Conduct differential expression analysis to find marker genes.
• Annotate cell types using known markers or automated tools.
• Hands-on Q&A and Discussion
• Generate insightful visualizations, including:
• UMAP/t-SNE plots for cell clustering
• Feature plots, violin plots, and dot plots for gene expression analysis
• Heatmaps for differentially expressed genes
• Integrate results into RMarkdown for reproducible reporting.

Requirements:

Laptop is required for this hands-on workshop with R ≥4.0, RStudio, and Seurat installed

Basic knowledge of R and RStudio is recommended.

Before attending, please install the following R packages:

• Seurat
• dplyr
• ggplot2

Scripts and example datasets will be provided before the workshop

Target Audience:

This workshop is ideal for biologists, bioinformaticians, and computational researchers who want to gain hands-on experience with single-cell RNA-seq analysis. Whether you are new to scRNA-seq or looking to refine your skills, this session will provide practical guidance for working with Seurat.

Registration is required to attend.