Bioinformatics workshop series: 1. Foundations of Bulk-RNAseq Library Preparation
Bioinformatics workshop series: 1. Foundations of Bulk-RNAseq Library Preparation In-Person
Session 1 – Foundations of Bulk-RNAseq Library Preparation
Registration required* and In-Person
Presenter: Bony De Kumar, Yale Center for Genomic Analysis
Series Details: see below
Summary: Explore the core principles and techniques of bulk-RNAseq library preparation. This introductory session lays the groundwork for understanding the complexities of bulk RNA-seq experiments.
In this talk, Dr. Bony De Kumar, Director of operations at the Yale Center for Genome Analysis will review Basic aspects of gene expression analysis. This session will elaborate on various types of RNA-Seq methods and their applications.
You will learn how to:
- Different approaches to sequence RNA
- Various methods of RNA-Seq library preparation and their downstream application
- RNA Quality requirements
- Full length RNA Sequencing approaches and epitranscriptomics
Requirements:
None
This session is suitable for both beginner, intermediate and advanced users.
- Date:
- Tuesday, February 6, 2024
- Time:
- 10:00am - 12:00pm
- Time Zone:
- Eastern Time - US & Canada (change)
- Location:
- SHM L E26/E27, Cushing/Whitney Medical Library, 333 Cedar St
- Campus:
- Medical School
- Categories:
- Bioinformatics
Bioinformatics workshop series: Intro to bulk RNA-seq data analysis: from experimental design to pathway analysis
Registration required* and In-Person
GENERAL DESCRIPTION: This workshop series offers an introduction to bulk RNA-seq data analysis, guiding participants through each step, from library preparation to gene set enrichment analysis. The series is composed of 8 sessions (see table below). The sessions will be led by field experts from the Yale Centers for Genomic Analysis (YCGA), Computational Research (YCRC) and Cushing/Whitney Medical Library Bioinformatics Support Hub to better connect you with bioinformatics services available at Yale.
We have structured this series in such a manner as to provide practical skills in experimental design, data processing, and functional analysis. The series is ideal for those new to the field of transcriptomics or those wanting to start performing their own analysis.
Date: Every Tuesday from February 6 until March 26
Time: from 10am-12pm
Requirements: No previous command line experience required.
Seating Information: For registered participants, seating operates on a first-come, first-served basis. Please ensure timely arrival to secure your seat.
*Registrations for March sessions will open at the end of February.
|
DATE |
SESSION |
MODE |
PRESENTER |
|
2/06/24 |
Session 1 - Foundations of Bulk-RNAseq Library Preparation |
In-person |
Bony De Kumar, PhD |
|
2/13/24 |
Session 2 - Considerations for Designing Next-Generation Sequencing Experiments |
In-person |
Francesc Lopez Giraldez, PhD |
|
2/20/24 |
Session 3 - Introduction to the Yale Clusters and Open OnDemand |
In-person |
Robert Bjornson, PhD |
|
2/27/24 |
Session 4 - RNAseq Data Processing: A Step-by-Step Guide from raw sequences to counts |
In-person |
Francesc Lopez Giraldez, PhD |
|
3/05/24 |
Session 5 – Streamlining RNAseq Analysis with the YCGA Pipeline |
In-person |
Francesc Lopez Giraldez, PhD |
|
3/12/24 |
Session 6 – Analysis of differential gene expression of bulk RNA-seq data using “DESeq2” in RStudio. |
In-person |
Sofia Fertuzinhos, PhD |
|
3/19/24 |
Session 7 - Functional analysis using R |
In-person |
Bony De Kumar, PhD |
|
3/26/24 |
Session 8 - Functional analysis using Gene Set Enrichment Analysis (GSEA) |
In-person |
Rolando Milian, MLS |
